Stacks of spatial data can be animated and measured, but you can also use ImageJ to construct entirely new views of features in the images. Stacking temporal images allows you to animate them � to rapidly display them in sequence � making changes over time easier to see and understand, and allowing you to precisely measure the same regions of the image over time. Stacks are used to display and analyze images that are related to each other in some way, such as by time (temporal), space (spatial), or color (spectral). The number and size of the images you can stack depend on the amount of memory in your computer. To stack a set of images, they must all be the same width, height, and bit depth. Many operations, such as selecting, filtering, thresholding, and contrast enhancement can be applied to all slices in a stack. Stack windows have a scroll bar across the bottom to cycle through the slices, and you can animate the images at a speeds from one frame every 10 seconds to over 1000 frames per second. The images or layers that make up a stack are called slices. ImageJ can display two or more images in a single window, as a stack. Part 2: Stack and Animate Time-Series Images 1 2 Introduction to ImageJ Stacks
